science | 2019

A cytosine deaminase for programmable single-base RNA editing

Omar O Abudayyeh, Jonathan S Gootenberg, Brian Franklin, Jeremy Koob, Max J Kellner, Alim Ladha, Julia Joung, Paul Kirchgatterer, David BT Cox, Feng Zhang

The crispr journal | 2019

Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13

Omar O Abudayyeh, Jonathan S Gootenberg, Max J Kellner, Feng Zhang

nature comm | 2019

Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9

Seiichi Hirano, Omar O Abudayyeh, Jonathan S Gootenberg, Takuro Horii, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Hiroshi Nishimasu, Osamu Nureki

cell reports | 2019

High-resolution structure of Cas13b and biochemical characterization of RNA targeting and cleavage

Ian M Slaymaker, Pablo Mesa, Max J Kellner, Soumya Kannan, Edward Brignole, Jeremy Koob, Patricia R Feliciano, Stefano Stella, Omar O Abudayyeh, Jonathan S Gootenberg, Jonathan Strecker, Guillermo Montoya, Feng Zhang

Science | 2018

Engineered CRISPR-Cas9 nuclease with expanded targeting space

Hiroshi Nishimasu, Xi Shi, Soh Ishiguro, Linyi Gao, Seiichi Hirano, Sae Okazaki, Taichi Noda, Omar O Abudayyeh, Jonathan S Gootenberg, Hideto Mori, Seiya Oura, Benjamin Holmes, Mamoru Tanaka, Motoaki Seki, Hisato Hirano, Hiroyuki Aburatani, Ryuichiro Ishitani, Masahito Ikawa, Nozomu Yachie, Feng Zhang, Osamu Nureki

Science | 2018

Field-deployable viral diagnostics using CRISPR-Cas13

Cameron Myhrvold, Catherine A Freije, Jonathan S Gootenberg, Omar O Abudayyeh, Hayden C Metsky, Ann F Durbin, Max J Kellner, Amanda L Tan, Lauren M Paul, Leda A Parham, Kimberly F Garcia, Kayla G Barnes, Bridget Chak, Adriano Mondini, Mauricio L Nogueira, Sharon Isern, Scott F Michael, Ivette Lorenzana, Nathan L Yozwiak, Bronwyn L MacInnis, Irene Bosch, Lee Gehrke, Feng Zhang, Pardis C Sabeti

Science | 2018

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S Gootenberg, Omar O Abudayyeh, Max J Kellner, Julia Joung, James J Collins, Feng Zhang

nature comm | 2018

Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

Josh Tycko, Luis A Barrera, Nicholas C Huston, Ari E Friedland, Xuebing Wu, Jonathan S Gootenberg, Omar O Abudayyeh, Vic E Myer, Christopher J Wilson, Patrick D Hsu

Science | 2017

RNA editing with CRISPR-Cas13

David BT Cox, Jonathan S Gootenberg, Omar O Abudayyeh, Brian Franklin, Max J Kellner, Julia Joung, Feng Zhang

Nature | 2017

RNA targeting with CRISPR–Cas13

Omar O Abudayyeh, Jonathan S Gootenberg, Patrick Essletzbichler, Shuo Han, Julia Joung, Joseph J Belanto, Vanessa Verdine, David BT Cox, Max J Kellner, Aviv Regev, Eric S Lander, Daniel F Voytas, Alice Y Ting, Feng Zhang

nature | 2017

Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood

Julia Joung, Jesse M Engreitz, Silvana Konermann, Omar O Abudayyeh, Vanessa K Verdine, Francois Aguet, Jonathan S Gootenberg, Neville E Sanjana, Jason B Wright, Charles P Fulco, Yuen-Yi Tseng, Charles H Yoon, Jesse S Boehm, Eric S Lander, Feng Zhang

science | 2017

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S Gootenberg, Omar O Abudayyeh, Jeong Wook Lee, Patrick Essletzbichler, Aaron J Dy, Julia Joung, Vanessa Verdine, Nina Donghia, Nichole M Daringer, Catherine A Freije, Cameron Myhrvold, Roby P Bhattacharyya, Jonathan Livny, Aviv Regev, Eugene V Koonin, Deborah T Hung, Pardis C Sabeti, James J Collins, Feng Zhang

nature protocols | 2017

Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

Julia Joung, Silvana Konermann, Jonathan S Gootenberg, Omar O Abudayyeh, Randall J Platt, Mark D Brigham, Neville E Sanjana, Feng Zhang

molecular cell | 2017

Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems

Mari Yamada, Yuto Watanabe, Jonathan S Gootenberg, Hisato Hirano, F Ann Ran, Takanori Nakane, Ryuichiro Ishitani, Feng Zhang, Hiroshi Nishimasu, Osamu Nureki

Nature rev microbio | 2017

Diversity and evolution of class 2 CRISPR–Cas systems

Sergey Shmakov, Aaron Smargon, David Scott, David Cox, Neena Pyzocha, Winston Yan, Omar O Abudayyeh, Jonathan S Gootenberg, Kira S Makarova, Yuri I Wolf, Konstantin Severinov, Feng Zhang, Eugene V Koonin

molecular cell | 2017

Cas13b is a type VI-B CRISPR-associated RNA-guided RNase differentially regulated by accessory proteins Csx27 and Csx28

Aaron A Smargon, David BT Cox, Neena K Pyzocha, Kaijie Zheng, Ian M Slaymaker, Jonathan S Gootenberg, Omar A Abudayyeh, Patrick Essletzbichler, Sergey Shmakov, Kira S Makarova, Eugene V Koonin, Feng Zhang

Nature biotechnology | 2017

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

Bernd Zetsche, Matthias Heidenreich, Prarthana Mohanraju, Iana Fedorova, Jeroen Kneppers, Ellen M DeGennaro, Nerges Winblad, Sourav R Choudhury, Omar O Abudayyeh, Jonathan S Gootenberg, Wen Y Wu, David A Scott, Konstantin Severinov, John Van Der Oost, Feng Zhang

Science | 2016

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Omar O Abudayyeh, Jonathan S Gootenberg, Silvana Konermann, Julia Joung, Ian M Slaymaker, David BT Cox, Sergey Shmakov, Kira S Makarova, Ekaterina Semenova, Leonid Minakhin, Konstantin Severinov, Aviv Regev, Eric S Lander, Eugene V Koonin, Feng Zhang

Cell | 2016

Structure and engineering of Francisella novicida Cas9

Hisato Hirano, Jonathan S Gootenberg, Takuro Horii, Omar O Abudayyeh, Mika Kimura, Patrick D Hsu, Takanori Nakane, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Hiroshi Nishimasu, Osamu Nureki

Molecular cell | 2015

Discovery and functional characterization of diverse class 2 CRISPR-Cas systems

Sergey Shmakov, Omar O Abudayyeh, Kira S Makarova, Yuri I Wolf, Jonathan S Gootenberg, Ekaterina Semenova, Leonid Minakhin, Julia Joung, Silvana Konermann, Konstantin Severinov, Feng Zhang, Eugene V Koonin

Nature biotechnology | 2015

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

James E Dahlman, Omar O Abudayyeh, Julia Joung, Jonathan S Gootenberg, Feng Zhang, Silvana Konermann

Cell | 2015

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Bernd Zetsche, Jonathan S Gootenberg, Omar O Abudayyeh, Ian M Slaymaker, Kira S Makarova, Patrick Essletzbichler, Sara E Volz, Julia Joung, John van der Oost, Aviv Regev, Eugene V Koonin, Feng Zhang

nature | 2015

In vivo genome editing using Staphylococcus aureus Cas9

F Ann Ran, Le Cong, Winston X Yan, David A Scott, Jonathan S Gootenberg, Andrea J Kriz, Bernd Zetsche, Ophir Shalem, Xuebing Wu, Kira S Makarova, Eugene V Koonin, Phillip A Sharp, Feng Zhang

Nature | 2015

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Silvana Konermann, Mark D Brigham, Alexandro E Trevino, Julia Joung, Omar O Abudayyeh, Clea Barcena, Patrick D Hsu, Naomi Habib, Jonathan S Gootenberg, Hiroshi Nishimasu, Osamu Nureki, Feng Zhang

Cell | 2013

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

F Ann Ran, Patrick D Hsu, Chie-Yu Lin, Jonathan S Gootenberg, Silvana Konermann, Alexandro E Trevino, David A Scott, Azusa Inoue, Shogo Matoba, Yi Zhang, Feng Zhang